NGS Automation: Streamline Your Lab Workflow Today
Next-generation sequencing (NGS) has revolutionized healthcare research, offering groundbreaking insights into the genome and driving innovations in precision medicine and diagnostics1. Labs worldwide are embracing laboratory automation to optimize their NGS workflows, tackling the challenges of manual sample preparation and clean-up that often lead to prolonged processes1.
Cutting-edge automation tools are transforming the NGS landscape. The I.DOT Liquid Handler and G.PURE NGS Clean-Up Device can automate up to 80% of the workflow, freeing up scientists’ time for analysis, boosting throughput, and improving data consistency1. These advancements are game-changers for labs aiming to enhance efficiency and reduce manual errors.
Workflow optimization through automation offers numerous benefits. It minimizes waste, promotes sustainability, and contributes to more resilient laboratory practices1. For DNA sequencing, fully automated systems like the NGS DreamPrep solution can prepare and quantify libraries in less than four hours, a significant time-saving compared to seven hours for other systems2.
By embracing NGS automation, labs can overcome common challenges such as inconsistent library concentrations and user-to-user variability2. The result? Higher quality data, improved reproducibility, and a streamlined path to groundbreaking discoveries.
Key Takeaways
- NGS automation enhances efficiency and reduces manual errors
- Automated tools can handle up to 80% of the NGS workflow
- Time savings of up to 3 hours in DNA sequencing library prep
- Improved data consistency and quality through automation
- Reduced waste and increased sustainability in lab practices
- Enhanced reproducibility and standardization of results
What is NGS Automation?
Next-generation sequencing (NGS) automation revolutionizes genetic analysis. It combines cutting-edge technology with streamlined processes to enhance research efficiency. Labs can now process vast amounts of genetic data faster and more accurately than ever before.
Understanding NGS Technology
NGS, also known as bulk parallel sequencing, allows for simultaneous analysis of multiple DNA fragments. This technology has transformed genomic research, enabling scientists to study genomes with unprecedented accuracy and. NGS platforms can process 4-384 samples per run, offering flexibility in scaling operations3.
The Role of Automation in NGS
Automated sample preparation is crucial in NGS workflows. It reduces manual labor, minimizes errors, and increases throughput. The time to set up an automated instrument for sequencing library preparation plus run time is approximately 30 minutes versus 3+ hours for manual processing per 8 samples3. This efficiency boost is driving the growth of the multi-billion dollar laboratory automation market.
| Aspect | Manual Process | Automated Process |
|---|---|---|
| Processing Time (8 samples) | 3+ hours | 30 minutes |
| Error Rate | Higher | Lower |
| Throughput | Limited | High (4-384 samples/run) |
| Cost per Sample | Variable | ~$40 |
NGS automation systems, like those offered by Illumina, streamline workflows from sample preparation to data analysis. These platforms integrate various steps, including PCR-based workflows and library preparation, enhancing overall efficiency in genomic research and clinical applications4.
Benefits of NGS Automation
NGS automation brings game-changing advantages to labs. By streamlining processes, it boosts productivity and enhances data quality. Let’s explore the key benefits that make NGS automation a must-have for modern laboratories.
Enhanced Throughput and Efficiency
Automated systems revolutionize high-throughput processing in NGS. They can handle larger sample volumes and reduce hands-on time significantly. Automated liquid handling can cut up to 80% of the NGS library preparation process, resulting in major savings in equipment, labor, and reagent costs5. The I.DOT Non-contact Dispenser supports dispensing volumes as low as 8 nL with only 1 µL dead volume, enabling cost-effective scaling of NGS reaction volumes5.
Improved Accuracy and Consistency
NGS automation enhances reproducibility by minimizing human errors. The I.DOT system reduces manual steps, lowering variability and preventing cross-contamination between samples5. This leads to fewer repeats due to errors, ensuring more consistent and reliable results. Automation also improves sample quality, a key factor in consistent library preparation outcomes6.
Time and Cost Savings
Workflow optimization through automation yields significant time and cost benefits. Manual NGS library preparation can take up to eight hours of hands-on time, while automation drastically reduces turnaround times5. The global NGS market is expected to grow at a 15.7% annual rate, reaching $27 billion by 2027, driven by these efficiencies7. Lab managers find that investing in automation outweighs initial costs due to long-term operational efficiency gains and reduced consumable expenses5.
| Aspect | Manual Process | Automated Process |
|---|---|---|
| Hands-on Time | Up to 8 hours | Significantly reduced |
| Error Rate | Higher | Lower |
| Sample Throughput | Limited | Increased |
| Cost Efficiency | Lower | Higher |
By freeing up researchers’ time, automation allows for more focus on data analysis and speeds up the delivery of innovations like personalized treatments in oncology7. The benefits of NGS automation make it an essential tool for labs aiming to stay competitive in the rapidly evolving field of genomics.
Key Features of NGS Automation Systems
NGS automation systems have transformed genetic analysis since their commercial introduction in 20058. These systems boast powerful features that streamline lab workflows and enhance research outcomes.
Sample Preparation Automation
Automated sample preparation is a game-changer in NGS workflows. It can reduce manual labor by up to 80%, leading to significant cost savings5. Advanced liquid handling systems like the I.DOT Non-contact Dispenser support dispensing volumes as low as 8 nL, minimizing reagent use and cutting costs5. This precision in NGS library preparation ensures consistent data quality and minimizes human errors9.
Data Management Integration
Effective data integration is crucial in NGS automation. These systems can process up to 96 samples simultaneously, reducing processing time and batch-to-batch variations9. This high throughput enables researchers to tackle novel questions across various fields, from drug discovery to environmental monitoring9. Whole genome analysis benefits greatly from these advancements, allowing for better detection of genomic rearrangements and RNA gene fusions.
Equipment Compatibility
NGS automation systems offer broad equipment compatibility. They support multiple plate formats and sequencing applications, seamlessly integrating into existing lab setups. This flexibility allows for paired-end and mate-pair library preparations, crucial for complex genomic studies8. While the initial investment in automation might seem high, the long-term benefits in time saved, reduced material losses, and increased walk-away time for researchers result in significant cost savings9.
| Feature | Benefit |
|---|---|
| Automated liquid handling | Reduces manual labor by 80% |
| High-throughput processing | Up to 96 samples simultaneously |
| Precision dispensing | As low as 8 nL volumes |
| Equipment compatibility | Supports multiple plate formats |
Popular NGS Automation Solutions
The field of Next Generation Sequencing (NGS) has seen rapid advancements in automation solutions. These innovations aim to streamline workflows and enhance efficiency in laboratories.
Overview of Leading Brands
INTEGRA Biosciences stands out with its MIRO CANVAS microfluidics platform, designed for high-precision liquid handling. The I.DOT Liquid Handler, another notable product, offers non-contact dispensing for accurate sample preparation. These systems cater to various NGS applications, from DNA library preparation to target enrichment10.
Many NGS automation solutions now incorporate enzymatic fragmentation, with 50% of DNA library prep systems using this advanced technique10. This trend reflects the industry’s move towards more efficient and gentle sample processing methods.
Comparison of Features and Pricing
While specific pricing information varies, NGS automation solutions focus on cost-effectiveness. For instance, miniaturization techniques can reduce library preparation reagent volumes by up to 10 times, allowing labs to generate multiple replicates at lower costs11.
Feature-wise, modern systems like the MIRO CANVAS offer protocol creation tools and automated clean-up processes. Some kits, such as Collibri DNA Library Prep Kits, provide even genomic coverage from as little as 1 ng of input, showcasing the sensitivity of current technologies11.
| Feature | MIRO CANVAS | I.DOT Liquid Handler |
|---|---|---|
| Dispensing Method | Microfluidics | Non-contact |
| Protocol Creation | Yes | Yes |
| Automated Clean-up | Yes | No |
The diversity in NGS automation solutions caters to various lab needs. For example, AmpliSeq chemistry offers highly multiplexed PCR-based workflows for targeting a few to hundreds of genes in a single run, while TruSeq DNA Nano efficiently generates whole-genome sequencing libraries4. This range of options allows labs to choose systems that best fit their specific research goals and budget constraints.
Implementing NGS Automation in Your Lab
Embracing NGS automation can revolutionize your lab’s efficiency. A thorough workflow assessment is crucial before diving into automation implementation. Labs that automate up to 80% of their NGS library preparation process can significantly reduce equipment, labor, and reagent costs5.
Assessing Your Current Workflow
Start by evaluating your lab’s specific needs. Consider sample types, throughput requirements, and existing equipment. Automated platforms can process 4 to 384 samples per run, offering scalability for various lab sizes12. This flexibility allows for gradual automation adoption, enhancing laboratory efficiency over time.
Selecting the Right Tools
Choose automation tools that align with your lab’s goals. Automated liquid handlers improve sample quality and consistency, reducing failed runs12. The I.DOT Non-contact Dispenser, for example, supports volumes as low as 8 nL with only 1 µL dead volume, enabling cost-effective miniaturization5.
| Automation Feature | Benefit |
|---|---|
| Automated liquid handling | Improved reproducibility, error prevention |
| LIMS integration | Streamlined data transfer, reduced manual recording |
| Custom workflows | Optimized efficiency, automated decision-making |
Training and Support Considerations
Proper training is essential for successful automation implementation. Manufacturers often provide on-site familiarization, but additional hands-on experimentation is necessary for smooth workflow integration12. Regular maintenance following manufacturer guidelines ensures optimal performance and longevity of your automated systems.
By carefully assessing your workflow, selecting appropriate tools, and investing in training, you can harness the full potential of NGS automation to boost your lab’s efficiency and output quality.
Overcoming Common Challenges
Implementing NGS automation brings its share of hurdles. Labs face technical issues and staff resistance when adopting new systems. Let’s explore ways to tackle these obstacles.
Addressing Technical Difficulties
Troubleshooting is key when dealing with NGS automation challenges. Technical glitches can slow down workflows and impact data quality. One major issue is library preparation inefficiency, which can affect the reliability of results13. To combat this, labs can use specialized kits that reduce workflow steps and minimize sample loss14.
Automated liquid handlers, like the I.DOT Liquid Handler from DISPENDIX, can streamline processes and reduce errors in NGS workflows13. These systems help maintain a low signal-to-noise ratio, crucial for distinguishing true genetic variants from errors13.
Ensuring Staff Buy-In
Staff training is vital for successful automation adoption. Clear communication about the benefits of NGS automation can help gain staff support. For example, automated systems can prepare samples quickly and at scale, benefiting both large and small labs with limited resources14.
To illustrate the impact of automation, consider this comparison:
| Process | Manual | Automated |
|---|---|---|
| Sample Preparation Time | Several hours | Minutes to hours |
| Error Rate | Higher | Lower |
| Throughput | Limited | High |
| Consistency | Variable | Highly consistent |
By showcasing these benefits, labs can foster enthusiasm for NGS automation adoption. With proper training and support, staff can harness the full potential of automated systems, leading to more efficient and accurate genetic analysis.
Future Trends in NGS Automation
The future of sequencing is bright, with NGS automation set to revolutionize genomics research. As technology advances, we’re seeing incredible growth in this field. The NGS Automation Market is projected to grow at a CAGR of 13.4% from 2022 to 2029, reaching $1.33 billion by 202915. This rapid expansion signals exciting developments on the horizon.
Advances in Technology
NGS platforms are evolving to meet growing demands. Advanced systems like Illumina, Pacific Biosciences, and Oxford Nanopore now enable parallel sequencing of millions to billions of DNA fragments16. These improvements are driving faster, more accurate sequencing at lower costs.
Whole genome sequencing is leading the charge, estimated to have the largest market share in NGS automation15. This technology provides detailed information about genomes, genetic variations, and gene behavior, opening new doors in research and medicine16.
Predictive Analytics and Machine Learning
AI in genomics is transforming data analysis. Machine learning for NGS is enhancing our ability to interpret complex genetic information. These tools are particularly valuable in drug discovery, which is forecasted to hold the largest share of the NGS automation market15.
| Region | Growth Rate | Key Segment | Market Share |
|---|---|---|---|
| Asia-Pacific | 14.7% | Workstation/Robotic Platform | 73% |
| Global | 13.4% | Pharmaceutical & Biotechnology Companies | 54% |
As we look to the future, NGS automation is poised to make significant strides in personalized medicine and diagnostic applications. With Asia-Pacific emerging as the fastest-growing market and product launches driving innovation, the landscape of genomic research is set for exciting transformations15.
Case Studies of Successful NGS Automation
NGS automation case studies reveal impressive advancements in research applications and clinical genomics. These real-world examples showcase how labs are transforming their workflows and achieving remarkable results.
Academic Research Lab Success
The adoption of NGS automation in academic settings has led to groundbreaking discoveries. At Queen’s University Belfast, researchers implemented the AVENIO Edge System for automated NGS library preparation in cancer sample analysis. The system proved easy to adopt, even for non-automation-savvy technicians, and delivered excellent correlation between manual and automated preparation results17.
In another study, a lab-on-a-chip platform demonstrated highly comparable results to manual processing, with a Pearson correlation coefficient of 0.94 for amplicon sequencing18. This breakthrough showcases the potential of automated systems in producing reliable, high-quality data for clinical genomics research.
Clinical Laboratory Implementation
Clinical labs have seen significant improvements in efficiency and accuracy through NGS automation. The close collaboration between university and clinical research laboratories in Northern Ireland provides evidence for the rapid adoption of NGS in genetic testing17. This partnership has accelerated the integration of automated workflows in diagnostic settings.
The introduction of the Vivalytic lab-on-a-chip platform in 2020 marked a new era in clinical genomics automation. With its 12 heaters for microfluidic control and four-layer cartridge design, this system offers a compact solution for high-throughput processing18. Such advancements are crucial in meeting the growing demand for NGS automation in clinical applications.
These case studies highlight the transformative impact of NGS automation across both academic and clinical settings, paving the way for more efficient and accurate genomic analysis.
Conclusion: Embracing NGS Automation
NGS automation has emerged as a game-changer in modern laboratories. It tackles common challenges like pipetting errors and human mistakes that can render data unusable19. This shift towards automation is reshaping the landscape of genomic research and clinical diagnostics.
Final Thoughts on Workflow Optimization
The benefits of NGS workflow optimization are clear. Automated systems can handle up to 80% of the NGS workflow, boosting throughput and data quality19. Tools like the I.DOT Liquid Handler cut costs by minimizing reagent use and reducing the need for pipette tips19. These laboratory automation benefits extend beyond mere efficiency. They free up scientists to focus on analysis and innovation, paving the way for groundbreaking discoveries19.
Public health labs are increasingly adopting NGS technologies, especially for foodborne pathogen surveillance. This trend is expected to expand to other public health applications20. However, challenges remain. Many labs lack the IT infrastructure and expertise needed for NGS applications, highlighting the need for workforce education in bioinformatics20.
Take the Next Step
As genomic research advancements continue, embracing NGS automation is crucial for staying competitive. The transition might seem daunting, but the long-term gains in efficiency, cost savings, and data reliability are worth the initial investment19. Whether you’re in a clinical setting or academic research, now is the time to explore how NGS automation can transform your lab’s capabilities and contribute to the next wave of scientific breakthroughs.
Q&A
What is NGS automation?
NGS automation is the use of advanced technologies to streamline the next-generation sequencing workflow, from sample preparation to data analysis. It involves using automated devices to handle critical steps such as mixing, PCR, and bead cleanup, reducing manual errors and improving overall efficiency.
What are the benefits of NGS automation?
The benefits of NGS automation include increased throughput and productivity, improved consistency and data quality, and significant time and cost savings. Automated systems can process larger sample volumes, reduce hands-on time, and minimize reagent use, allowing scientists to focus more on data analysis and discovery.
What are some key features of NGS automation systems?
Key features of NGS automation systems include automated sample preparation, integrated data management, and compatibility with various equipment. Many systems offer batch processing, minimal dead volume, and support for multiple plate formats and sequencing applications, enabling seamless integration into existing laboratory workflows.
Can you name some popular NGS automation solutions?
Popular NGS automation solutions include INTEGRA Biosciences’ MIRO CANVAS microfluidics platform, the I.DOT Liquid Handler, and the G.PURE NGS Clean-Up Device. These systems offer features such as non-contact dispensing, protocol creation tools, and automated clean-up processes.
How do I implement NGS automation in my lab?
Implementing NGS automation requires assessing your current workflows, selecting appropriate tools, and considering training and support needs. Evaluate your specific requirements, such as throughput needs and sample types. Choose automation solutions by comparing features like dispensing accuracy, compatibility with existing equipment, and scalability.
What are some common challenges in NGS automation?
Common challenges in NGS automation include technical difficulties during implementation or operation of automated systems and ensuring staff buy-in. These can be addressed through proper training, ongoing support from vendors, clear communication, and involvement of laboratory staff in the implementation process.
What are the future trends in NGS automation?
Future trends in NGS automation include advances in technology, integration of predictive analytics and machine learning. Emerging technologies may further streamline workflows and improve data accuracy. Machine learning algorithms could enhance data analysis and interpretation, potentially revolutionizing personalized medicine and diagnostic applications.
How much of the NGS workflow can be automated?
Automation can handle up to 80% of the NGS workflow, significantly reducing turnaround times and allowing scientists to focus more on analysis and discovery rather than repetitive manual tasks.
What is the MIRO CANVAS microfluidics platform?
The MIRO CANVAS microfluidics platform is an automated device from INTEGRA Biosciences that simplifies NGS workflows. It helps save time, minimize reagent use, and improve experimental flexibility in next-generation sequencing processes.
How does the I.DOT Liquid Handler contribute to NGS automation?
The I.DOT Liquid Handler is an automated device that contributes to NGS automation by offering features such as batch processing, DropDetection technology, and minimal dead volume. It helps in critical steps of the NGS workflow, improving efficiency and reducing the chances of human error.
