Illumina NovaSeq 6000 Price: What You Need to Know
The Illumina NovaSeq 6000 is a game-changer in high-throughput sequencing. This powerful Illumina sequencing system, released in March 2017, offers unparalleled speed and efficiency for genomic research1.
With an initial price tag of $985,000, the NovaSeq 6000 represents a significant investment for labs and research institutions1. However, recent market shifts have seen the cost drop to around $350,000, making this cutting-edge technology more accessible2.
The NovaSeq 6000 boasts impressive specs. It weighs 481 kg and requires 200-240 VAC power supply2. Its throughput is remarkable, delivering up to 6 Tb and 20 billion reads per dual S4 run2.
Cost-conscious researchers will appreciate the range of sequencing options. Prices span from $125 per sample for basic sequencing to $17,500 per cell for the most advanced NovaSeq S4 150 bp PE flow cell3. This flexibility allows labs to tailor their NovaSeq 6000 use to their specific needs and budgets.
Key Takeaways:
- NovaSeq 6000 initial cost was $985,000, now around $350,000
- Offers up to 6 Tb throughput per dual S4 run
- Various models available: S1, S2, S4, and SP
- Sequencing costs range from $125 to $17,500 per sample/cell
- Delivers high-quality data with ≥90% bases above Q30
Understanding the Illumina NovaSeq 6000 System
The Illumina NovaSeq 6000 System represents a leap forward in sequencing technology. This powerful platform combines advanced chemistry, optics, and informatics to deliver exceptional speed and data quality in genomic research4.
Overview of the NovaSeq 6000 Technology
The NovaSeq 6000 utilizes cutting-edge Illumina NGS techniques to streamline operations and provide insights into genomics that were previously challenging to uncover4. Its patterned flow cells with nanowells ensure precise cluster spacing, enhancing sequencing accuracy.
This system offers impressive throughput, with the ability to sequence up to 64 human whole genomes per flow cell for rare disease research5. The NovaSeq 6000 specifications include dual flow cell mode and high-resolution imaging, allowing for flexible experimental design.
Key Features and Benefits
The NovaSeq 6000 boasts several advantages:
- High throughput: Up to 6 Tb output and 20B reads per dual S4 run
- Flexibility: Compatible with various library preparation kits
- Efficiency: Run times of approximately 17-48 hours depending on flow cell type5
- Scalability: Suitable for whole genome, exome, and RNA sequencing
The system’s optimized reagent kits improve cluster density, read length, and sequencing quality compared to earlier versions4. This enhancement allows researchers to efficiently identify coding variants by sequencing approximately 750 exomes per flow cell5.
| Feature | Benefit |
|---|---|
| Patterned flow cells | Precise cluster spacing |
| Dual flow cell mode | Increased experimental flexibility |
| 2-channel SBS chemistry | Improved sequencing speed and accuracy |
| High-resolution imaging | Enhanced data quality |
The NovaSeq 6000 System has revolutionized genomics studies since its launch, meeting customer demands and shaping possibilities for the future of genomics analysis4. Its advanced features and benefits make it a valuable tool for researchers across various fields of genomic research.
Factors Influencing NovaSeq 6000 Pricing
Understanding NovaSeq 6000 pricing factors is crucial for labs considering this advanced sequencing system. The cost of this Illumina instrument varies based on several key elements that impact both initial and ongoing expenses.
Initial Equipment Costs
The upfront cost of the NovaSeq 6000 is significant, reflecting its cutting-edge technology. While exact prices may vary, it’s important to note that Illumina has introduced the newer NovaSeq X series in 2023, priced at $985,000 MSRP as of February 20246. This gives an indication of the price range for high-end sequencing systems.
Pricing can be influenced by:
- Regional variations
- Bulk order discounts
- Warranty options
- Financing arrangements
Maintenance and Operational Expenses
Ongoing costs play a significant role in the total investment for the NovaSeq 6000. These include:
| Expense Type | Details |
|---|---|
| Reagent Costs | $5.53 to $12.09 per Gb, depending on flow cell type |
| Power Requirements | 200-240 VAC 50/60Hz, 16A, single phase, 2500 W |
| Maintenance Contracts | Essential for ongoing support and system optimization |
It’s worth comparing these costs to newer models. For instance, the NovaSeq X offers reagent costs ranging from $2.00 to $5.20 per Gb, potentially offering long-term savings6. Labs should consider these sequencing costs when evaluating their budget and expected usage.
When assessing the value of the NovaSeq 6000, consider its performance capabilities. For example, in SARS-CoV-2 variant analysis, a modified protocol on the MiniSeq instrument achieved 90% genome coverage for 142 out of 153 clinical samples, with a 99.34% accuracy rate in identifying variants7. While this data is for a different instrument, it illustrates the importance of considering both cost and performance in sequencing technology investments.
Breakdown of Illumina NovaSeq 6000 Price
The Illumina NovaSeq 6000 offers a range of models and configurations to suit different research needs. Understanding the pricing structure is crucial for labs considering this powerful sequencing system.
Pricing for Different Models
The NovaSeq 6000 system provides flexibility in read lengths and data output. It can produce 65-80 Gb for shorter reads up to 2400-3000 Gb for longer reads8. This versatility affects NovaSeq 6000 model prices, with costs varying based on the chosen configuration.
| Model | Read Length | Data Output | Estimated Price Range |
|---|---|---|---|
| SP | 2 x 50 bp | 65-80 Gb | $8,000 – $10,000 |
| S1 | 2 x 100 bp | 500-625 Gb | $12,000 – $15,000 |
| S2 | 2 x 150 bp | 1650-2000 Gb | $20,000 – $25,000 |
| S4 | 2 x 250 bp | 2400-3000 Gb | $30,000 – $35,000 |
Additional Costs to Consider
Beyond the initial equipment cost, labs must factor in sequencing reagent costs and Illumina flow cell pricing. For instance, library preparation services can range from $153 to $350 for affiliated investigators9. The NovaSeq 6000 Xp Workflow enables independent lane loading, potentially reducing costs for smaller projects4.
Run times vary from 13 to 44 hours depending on read length, impacting operational costs8. Labs should also consider maintenance, staff training, and data storage when budgeting for the NovaSeq 6000 system.
Comparing NovaSeq 6000 with Other Sequencing Options
When evaluating DNA sequencing platforms, it’s crucial to compare the NovaSeq 6000 with other options. This Illumina sequencer comparison helps researchers make informed decisions based on their specific needs and budget constraints.
Cost Comparison with Other Illumina Models
The NovaSeq 6000 offers high-throughput sequencing at competitive prices. For 150 PE sequencing with 40 million reads, the NovaSeq 6000 provides a Best Deal option at $125 per sample3. In contrast, the MiSeq sequencing options range from $795 to $2,250 per cell, while NextSeq 2000 options vary from $1,200 to $6,150 per cell3.
For larger projects, the NovaSeq S4 150 bp PE flow cell costs $17,500 for 10.0 billion reads across 4 independent lanes3. This pricing structure makes the NovaSeq 6000 cost-effective for high-volume sequencing needs.
Comparison with Competitor Sequencers
When comparing the NovaSeq 6000 to newer models like the NovaSeq X Series, we see significant advancements. The NovaSeq X Series allows sequencing of up to 64 human whole genomes per flow cell for rare disease research5. It also enables sequencing up to 2 million single cells per run with a 25B flow cell5.
The NovaSeq X Plus system with 25B flow cells powers exome sequencing and can sequence 2.5x more cells in half the time compared to the NovaSeq 60005. This improved efficiency makes the NovaSeq X Series a strong contender in the DNA sequencing platforms market.
| Feature | NovaSeq 6000 | NovaSeq X Series |
|---|---|---|
| Whole Genomes per Flow Cell | Up to 48 | Up to 64 |
| Single Cells per Run | Up to 800,000 | Up to 2 million |
| Exome Sequencing Speed | Standard | 2.5x faster |
While the NovaSeq 6000 remains a powerful and cost-effective option, researchers should consider their specific needs when choosing between different Illumina sequencer models and other DNA sequencing platforms.
Potential Discounts and Financing Options
Illumina offers various ways to make the NovaSeq 6000 more accessible for labs and academic institutions. These options can help manage costs and maximize budgets while staying competitive in the field of genomic research.
Available Discounts for Academic Institutions
NovaSeq 6000 academic pricing often includes special discounts for universities and research centers. These discounts can significantly reduce the upfront costs of acquiring this powerful sequencing system. Some institutions may even have access to tailored rental or leasing programs designed to work within their specific budget constraints10.
Financing Plans for Laboratories
Illumina financing options extend beyond academic settings. The company offers a reagent rental program that covers all products in countries where Illumina sells directly. This program helps labs maximize their budgets while staying competitive11. Lab equipment leasing is another popular option, allowing facilities to access cutting-edge technology without a large initial investment.
For labs needing flexibility, the NovaSeq 6000Dx instrument allows users to switch between RUO and IVD modes. This feature offers versatility for IVD testing, translational development, and clinical research on a single platform, potentially reducing overall equipment costs11.
When considering financing, it’s important to factor in the instrument’s reliability. Illumina provides remote monitoring and proactive support to maximize uptime, detect potential risks before running, and offer real-time updates. These features can help labs avoid unexpected costs and maintain efficient operations11.
Value Proposition of the NovaSeq 6000
The Illumina NovaSeq 6000 stands out as a game-changer in genomic research. Its high-throughput sequencing benefits and advanced features make it a top choice for labs seeking cutting-edge technology.
Why Invest in the NovaSeq 6000?
The NovaSeq 6000 offers unparalleled flexibility and scalability. Users can purchase flow cells in 2.5% increments, allowing cost-effective combination with other projects12. This system provides various flow cell options, ranging from 800 million to 10 billion reads, catering to diverse research needs12.
The NovaSeq 6000’s sequencing capacity is impressive, delivering over 20 billion paired-end reads per run13. This massive output enables processing of up to 1600 samples in a single run, showcasing its remarkable scalability13. Illumina system advantages shine through in the NovaSeq 6000’s performance, making it a valuable investment for high-volume sequencing labs.
Long-term Cost Savings Analysis
The NovaSeq 6000 ROI becomes evident when considering its efficiency and data quality. It produces significantly more read counts than other platforms, with an average of 193,081 compared to 71,406 on the MiSeq13. The system also boasts a higher percentage of non-chimeric reads (45.25%) and reads passing filters (86.50%)13.
For labs running multiple flow cells weekly, the NovaSeq 6000 offers a turnaround time of 4-6 weeks for samples sharing a 300-cycle S4 flow cell12. This efficiency, combined with its superior data quality and throughput, translates to substantial long-term cost savings and improved research outcomes.
User Experiences and Testimonials
The NovaSeq 6000 System has garnered praise from researchers worldwide for its exceptional performance in genomics research. This high-throughput sequencing platform offers scalable throughput and flexibility for various applications, helping users gain deeper insights into the genome4.
Feedback from Researchers Utilizing NovaSeq 6000
Researchers utilizing the NovaSeq 6000 have reported positive experiences with its advanced chemistry, optics, and informatics. These features combine to deliver outstanding speed, data quality, and throughput4. Many NovaSeq 6000 user reviews highlight the system’s ability to handle multiple projects simultaneously, making it a versatile tool for diverse research needs.
The optimized reagent kit has been a game-changer for many labs. It enhances cluster density and read length, resulting in improved sequencing quality scores compared to earlier versions4. This advancement has significantly boosted Illumina sequencer performance, allowing researchers to achieve more accurate and reliable results.
Case Studies of Successful Implementations
Numerous case studies showcase successful implementations of the NovaSeq 6000 in various genomics research experiences. The system’s support for comprehensive genomic profiling has been particularly valuable in cancer research. It enables the analysis of major variant classes and gene signatures from formalin-fixed paraffin-embedded tissue samples4.
One notable advancement is the NovaSeq 6000 v1.5 Reagent Kit, which aims to make whole-genome sequencing more accessible and affordable. With a target price of $600 per genome, this kit has opened new possibilities for large-scale genomic studies14. Researchers appreciate the extended shelf life of reagents, which has increased from three to six months, allowing for more efficient lab planning14.
The NovaSeq 6000 continues to evolve, supporting new library prep kits and assays. This flexibility, combined with Illumina’s innovative approach, has solidified its position as a leader in DNA sequencing technologies across research, clinical, and applied markets14.
Where to Purchase the Illumina NovaSeq 6000
Finding the right place to buy NovaSeq 6000 can be crucial for labs and research facilities. This powerful sequencing system offers exceptional speed and data quality, making it a valuable asset for genomic research4.
Authorized Distributors and Resellers
Illumina sequencer suppliers play a key role in distributing the NovaSeq 6000. These authorized partners ensure you receive genuine products with full manufacturer support. Many offer installation services, training, and ongoing technical assistance to help you maximize your investment.
Some genomics equipment retailers specialize in pre-owned systems. This option can provide cost savings while still delivering high-quality results. The NovaSeq 6000 is known for its scalability and outstanding throughput, making it a popular choice even in the secondary market4.
Online Purchase Options
Many Illumina sequencer suppliers offer online platforms to streamline the purchasing process. These sites often allow you to request quotes, compare models, and explore financing options. When you buy NovaSeq 6000 online, you can access detailed product information and specifications to make an informed decision.
Remember, the NovaSeq 6000 is designed for specific applications in the US, including targeted sequencing of DNA libraries from human genomic DNA extracted from blood or FFPE tissue10. In the EU and other regions, it’s intended for sequencing DNA libraries when used with IVD assays10. Always consult with the supplier about your specific research needs and regional regulations.
FAQs About NovaSeq 6000 Pricing
Curious about NovaSeq 6000 pricing FAQs? Let’s dive into some common questions and answers about this cutting-edge sequencing technology. The NovaSeq 6000 has revolutionized genomics studies since its launch, evolving to meet customer demands and shape the future of genomics4.
Common Questions and Answers
One frequently asked question is about the cost of the NovaSeq 6000. The machine is priced at $985,000, a significant investment for any lab15. Many wonder about the return on investment. With its exceptional speed and data quality, the NovaSeq 6000 can sequence 48 human genomes in just 48 hours, offering substantial time and cost savings164.
Another common query relates to Illumina sequencing costs. The cost for sequencing a human genome with Illumina’s highest-end products is currently $1,000, a dramatic decrease from the nearly 3 billion dollars spent on the Human Genome Project1516. This price reduction has made genomics research more accessible to a wider range of institutions.
Resources for Further Information
For more details on genomics equipment information, Illumina’s official website is a valuable resource. It provides in-depth information about the NovaSeq 6000’s optimized reagent kit, which offers increased cluster density and read length, enhancing sequencing quality scores4. Scientific publications and genomics research forums also offer comparative analyses of sequencing platforms, helping potential buyers make informed decisions.
Q&A
What is the base price of the Illumina NovaSeq 6000?
The Illumina NovaSeq 6000 had an MSRP of 5,000 upon its release in March 2017. However, actual prices may vary based on factors such as supplier, region, and potential discounts.
What are the different models of the NovaSeq 6000 available?
The NovaSeq 6000 is available in different models: S1, S2, S4, and SP. Each model offers different read lengths and run times to suit various research needs.
How much do NovaSeq 6000 reagents cost?
Reagent costs for the NovaSeq 6000 range from .53 to .09 per Gb, depending on the flow cell type used.
Are there any discounts available for academic institutions?
Yes, academic institutions may qualify for special pricing. For example, some universities offer discounted rates for NovaSeq 6000 services to their faculty and research investigators.
How does the NovaSeq 6000 compare to other Illumina sequencers in terms of cost?
The NovaSeq 6000 offers higher throughput compared to MiSeq and NextSeq models, which may result in lower per-sample costs for large-scale projects. However, it also has a higher initial investment.
Are there financing options available for purchasing a NovaSeq 6000?
Yes, financing options are available. Some suppliers offer leasing plans, and companies like REUZEit provide financing options for pre-owned NovaSeq 6000 systems, which can significantly reduce upfront costs.
What additional costs should I consider when budgeting for a NovaSeq 6000?
Additional costs to consider include warranty options, maintenance contracts, library preparation services, and operational expenses such as power consumption (2500 W).
How does the NovaSeq 6000 compare to competitor sequencers in terms of price and performance?
The NovaSeq 6000 competes with systems like the Ultima Genomics UG100 and Complete Genomics DNBSEQ-T7. While the UG100 offers faster run times, it has a higher upfront cost. The DNBSEQ-T7 provides similar read lengths but lower overall data output.
Where can I purchase a NovaSeq 6000?
The NovaSeq 6000 can be purchased directly from Illumina, through authorized distributors, or from specialized retailers offering pre-owned systems. Online platforms also provide options to request quotes or make inquiries about purchasing the system.
What is the output capacity of the NovaSeq 6000?
The NovaSeq 6000 offers up to 6 Tb output and 20 billion reads per dual S4 run, making it suitable for large-scale genomics projects.
