10x Genomics Support – Get Expert Help & Assistance
Welcome to the world of cutting-edge genomics! 10x Genomics offers comprehensive support for their revolutionary single-cell analysis platforms. Their expert help ensures you get the most out of your research. From technical queries to order placements, they’ve got you covered.
The Xenium In Situ platform allows high-throughput subcellular mapping of hundreds of RNA targets, supporting both fresh frozen and FFPE samples1. This versatility extends to Visium Spatial Gene Expression, which has been tested on over 30 different tissue types and featured in nearly 90 peer-reviewed articles2.
Need assistance? Reach out to [email protected] for technical support or [email protected] for orders. Their team of experts provides comprehensive support across North America, Europe, and Asia. For those who prefer self-help, 10x Genomics offers extensive FAQs and a Q&A Knowledgebase.
With Visium Spatial Gene Expression, you’ll enjoy high cellular resolution and compatibility with various staining techniques2. Plus, 10x Genomics provides free software tools like Space Ranger and Loupe Browser to aid in your data analysis2.
Key Takeaways
- Expert help available via email for technical support and orders
- Comprehensive support across multiple regions
- Self-help options include FAQs and Q&A Knowledgebase
- Xenium In Situ platform offers high-throughput RNA mapping
- Visium Spatial Gene Expression compatible with various tissues and staining techniques
- Free software tools provided for data analysis
Understanding 10x Genomics Technology
10x Genomics revolutionizes genomic research with cutting-edge platforms for single-cell analysis, spatial genomics, and in situ technology. Their innovative solutions empower scientists to explore cellular complexities at unprecedented levels.
Overview of 10x Genomics
10x Genomics offers a suite of powerful tools for genomic exploration. The Chromium Single Cell platform enables transcriptomic profiling of up to a million single cells with multiomic capabilities3. This technology has been utilized in over 8,200 published studies, highlighting its widespread adoption and impact in the scientific community3. The GEM-X technology reduces multiplet rates two-fold and increases throughput capabilities, enhancing the efficiency of single-cell analysis4.
Key Applications and Benefits
The applications of 10x Genomics technology span various fields of genomic research. The Single Cell Gene Expression Flex assay provides 3′ gene expression profiling at scale, while the Single Cell Immune Profiling offers 5′ gene expression alongside V(D)J repertoire profiling5. For spatial genomics, the Visium Spatial platform enables whole transcriptome analysis of FFPE & fresh frozen tissue, preserving crucial spatial context3. The Xenium Prime 5K assays push the boundaries of in situ technology, allowing single cell spatial imaging of 5,000 genes across tissue sections3.
These advanced technologies offer numerous benefits to researchers. Single cell RNA-seq provides high-resolution insights unmatched by other methods, allowing for the detection of novel subtypes and biomarkers3. The standardized workflow of the Chromium platform makes reliable, reproducible, and impactful single cell insights accessible to researchers from various fields4. With tools like Cell Ranger for data analysis and Loupe Browser for visualization, 10x Genomics provides a comprehensive ecosystem for genomic exploration5.
Types of Support Offered
10x Genomics provides comprehensive support to help researchers maximize their use of cutting-edge genomic technologies. The company offers various assistance channels to meet diverse user needs.
Technical Support Options
For technical assistance, 10x Genomics offers expert help through their Support Hub. This platform provides access to troubleshooting guides, software updates, and direct communication with specialists. Users can find solutions for issues related to Chromium Single Cell Gene Expression, which enables profiling of 2,000–20,000 cells per channel6.
Customer Service Inquiries
The customer service team at 10x Genomics handles inquiries about product orders, shipping, and general information. They assist with questions regarding the company’s platforms, including Chromium Single Cell, Visium Spatial, and Xenium In Situ technologies6.
Training and Resources
10x Genomics offers extensive training programs to ensure users can fully leverage their technologies. These include online resources, datasets, and publications. The company provides free access to publicly available datasets from various sample types, helping researchers explore real-world applications6. Training covers topics such as probe-based, 3′, and 5′ gene expression, as well as multiomic capabilities like cell surface protein identification and CRISPR screening assessment7.
| Support Type | Features |
|---|---|
| Technical Assistance | Support Hub, Troubleshooting Guides, Software Updates |
| Customer Service | Order Inquiries, Product Information, Shipping Assistance |
| Training Resources | Online Materials, Datasets, Publications, Multiomic Capabilities Training |
With these comprehensive support options, 10x Genomics ensures that researchers can effectively utilize their advanced genomic tools and platforms for groundbreaking scientific discoveries.
Getting Started with 10x Genomics
Starting your journey with 10x Genomics is an exciting step in advancing your genomic research. This section guides you through the initial setup and product selection process, ensuring you make the most of their cutting-edge technology.
Initial Setup Guidance
Setting up your 10x Genomics system requires careful planning and setup assistance. The Chromium Single Cell 3′ solution processes hundreds to thousands of single cells in a quick 7-minute run, with a cell recovery rate of up to 65%8. For optimal results, ensure accurate cell counting and maintain high cell viability, as these factors impact loading precision9.
Selection of Products and Kits
10x Genomics offers a range of genomics kits tailored to various research needs. The Single Cell Gene Expression solution measures 3′ gene expression for up to 20,000 cells per sample, while the Flex version can profile up to 1,024,000 fixed single cells across 16 samples in one run9. For immune research, the Single Cell Immune Profiling kit analyzes up to 20,000 cells per sample for immune repertoire and gene expression9.
| Product | Capacity | Application |
|---|---|---|
| Single Cell Gene Expression | Up to 20,000 cells/sample | 3′ gene expression analysis |
| Single Cell Gene Expression Flex | Up to 1,024,000 cells/16 samples | Fixed cell gene expression profiling |
| Single Cell Immune Profiling | Up to 20,000 cells/sample | Immune repertoire and gene expression |
When setting up your experiment, consider the dataset size and reference genome requirements. For example, a 1,000 PBMC dataset from human peripheral blood mononuclear cells is about 5.17G, while the Human reference GRCh38-2020-A genome file is 10.6G10. Plan your storage accordingly for smooth data processing and analysis.
Troubleshooting Common Issues
When using 10x Genomics technology, you might encounter various technical issues. Understanding how to troubleshoot these problems is crucial for maintaining smooth operations in your research.
Device Malfunctions
Device malfunctions can occur unexpectedly, disrupting your workflow. Preflight failures, often caused by invalid input data or runtime parameters, are the most common type of failure reported directly to the terminal1112. If you experience a device malfunction, check your input data and parameters first.
Software Errors
Software errors can be tricky to resolve. In-flight failures, typically caused by external factors like running out of system memory or disk space, may produce various error messages at different pipeline stages1112. Hard failures at different stages generate specific error messages, resulting in _errors files with detailed information about the cause of the halt1113.
Sample Preparation Problems
Sample prep issues can significantly impact your results. Alerts in web_summary.html files come in two levels: WARN for suboptimal parameters and ERROR for major issues11. ERROR alerts signify major problems that could lead to dubious results, such as using incorrect genome references1112.
If troubleshooting on your own doesn’t resolve the issue, contact the 10x Genomics software support team. They can provide expert guidance and may request data transfer via Redstone for further analysis121311.
Resources for Product Documentation
10x Genomics offers a wealth of product documentation to support users in their research endeavors. From user manuals to detailed protocols, these resources aim to maximize the potential of their cutting-edge genomic technologies.
Accessing User Manuals
User manuals for 10x Genomics products are readily available through their online Support Hub. These guides cover a range of platforms, including Chromium Single Cell, Visium Spatial, and Xenium In Situ5. Each manual provides step-by-step instructions for setup, operation, and maintenance, ensuring users can effectively utilize their chosen platform.
Finding Protocols and Guides
10x Genomics offers an extensive collection of protocols and guides tailored to various applications. These resources cover single-cell gene expression profiling, immune profiling, and spatial gene expression analysis5. The company provides free access to publicly available datasets, allowing researchers to explore real-world examples and refine their experimental designs6.
| Documentation Type | Content | Accessibility |
|---|---|---|
| User Manuals | Setup, operation, maintenance | Online Support Hub |
| Protocols | Application-specific methods | Downloadable PDFs |
| Guides | Best practices, troubleshooting | Interactive online format |
| Datasets | Sample data for various applications | Free public access |
The product documentation includes comprehensive support for software analysis, offering pipelines, visualization tools, and Cloud Analysis capabilities. These resources enable users to effectively analyze their single-cell data and gain valuable insights from their experiments6. By providing such extensive documentation, 10x Genomics empowers researchers to push the boundaries of genomic discovery.
Best Practices for Sample Preparation
Sample preparation plays a crucial role in achieving high-quality results with 10x Genomics technology. Proper techniques ensure accurate data and reliable outcomes in spatial transcriptomics and other genomic analyses.
Tips for High-Quality Samples
To obtain top-notch samples, start with accurate cell counting. Aim for 50-100 cells per square on a hemocytometer grid, with a standard deviation less than 25% for replicate counts14. Maintain cell stock concentration between 700-1,200 cells per µl and ensure cell viability exceeds 70%14.
Gentle handling is key. Mix cell suspensions 5-7 times with a pipette set to over 50% of the total volume14. Keep cells on ice and minimize preparation time. Remove dead cells and wash with PBS + 0.04% BSA to eliminate contaminants14.
Common Mistakes to Avoid
Avoid premature cell lysis by gentle pipette-mixing before loading onto microfluidic chips15. Don’t forget to aspirate cells from the middle of the sample when transferring to maintain accurate cell numbers15.
Neglecting to remove dead cells can impact targeted cell recovery rates15. Remember, different cell types have varying initial RNA content, which affects key decisions throughout the experimental design and workflow15.
- Always perform replicate cell counts and viability tests
- Adjust concentration if outside the optimal range
- Follow specific protocols available on the support site
By adhering to these best practices in sample preparation and quality control, you’ll set a strong foundation for successful genomic experiments.
Understanding Software Integration
10x Genomics offers powerful bioinformatics software for data analysis. Their tools streamline complex genomic data interpretation, making research more efficient.
10x Genomics Software Overview
The 10x Genomics Cloud Analysis platform is a game-changer for researchers. It simplifies and speeds up the interpretation of single-cell and spatial datasets. Users can easily run analysis pipelines and manage data effectively. The platform allows creating projects for each experiment and supports uploading different samples to a single project16.
Cloud Analysis comes with pre-built references for human, mouse, and barnyard datasets. Researchers can also upload custom references for their specific needs. The platform offers free usage policies and optional paid services, detailed in the Billing section16.
Integration with Third-Party Tools
Software integration is crucial in genomics research. Many experiments involve multiple samples, requiring robust data integration tools. The field benefits from community-developed tools that enhance software integration capabilities17.
To support researchers, numerous tutorials and guidelines for single-cell RNA-sequencing data analysis are available. These resources cater to both experimentalists and bioinformaticians, fostering a collaborative research environment. Various institutes offer courses and workshops to develop skills in single-cell data analysis17.
| Feature | 10x Genomics Cloud Analysis | Third-Party Tools |
|---|---|---|
| Data Integration | Built-in capabilities | Community-developed solutions |
| Reference Genomes | Pre-built and custom options | Varied, depends on tool |
| Learning Resources | Platform-specific tutorials | Diverse workshops and courses |
| Pricing Model | Free usage with paid options | Varies (open-source to commercial) |
Frequently Asked Questions
At 10x Genomics, we understand that users often have questions about our technology and services. Our comprehensive FAQs section addresses common queries and technical issues to help you navigate our products effectively.
General 10x Genomics Queries
Many users ask about the availability of Cloud Analysis. This service is accessible globally, with some exceptions. Cloud Analysis is not available in Cuba, Iran, North Korea, Syria, Russia, Belarus, China, Hong Kong, Venezuela, and Ukraine18. For optimal performance, we recommend using Chrome, Firefox, or Edge browsers when accessing Cloud Analysis18.
Technical Questions
Users often inquire about sample preparation. The recommended cell viability for submission is over 80%, with the number of cells submitted ranging from 1000 to 16,000 per sample19. Accurate cell counting is crucial for successful experiments. We suggest using automated cell counters or manual hemocytometers for precise results19.
Questions about project transfers are common. Each project can be transferred up to three times before this functionality is disabled18. Recipients of transferred projects in Cloud Analysis receive 90 days of free storage for each file, starting from the transfer completion date18.
Regarding timelines, the typical delivery for 10x Genomics experiments is 1-2 weeks19. We recommend submitting samples at least one week in advance, with larger experiments requiring earlier booking19. These FAQs aim to address common questions and technical queries, enhancing your experience with 10x Genomics technology.
Community Support and Forums
10x Genomics fosters a vibrant user community, promoting knowledge sharing and peer support. The company’s impact on genomics research is evident, with over 8,200 research papers citing their innovative technologies20. This expansive user base forms the foundation for a rich exchange of ideas and solutions.
Engaging with Other 10x Users
Users can tap into a wealth of expertise from peers worldwide. With 4,600+ instruments placed in top institutions globally, the potential for collaboration is immense20. Researchers can discuss best practices for sample preparation, aiming for the ideal cell concentration of 700-1,200 cells/uL with over 90% viability for optimal results21.
Sharing Knowledge and Solutions
The user community actively contributes to the growing body of genomics research. More than 7,000 publications have stemmed from 10x Genomics technologies, showcasing the collective knowledge of users20. Forums allow researchers to share tips on maximizing the Chromium Controller’s capacity, which can encapsulate up to 80,000+ individual cells in a single run21.
This collaborative environment enables users to troubleshoot issues, exchange protocols, and stay updated on the latest advancements. By leveraging the combined expertise of the community, researchers can optimize their use of 10x Genomics technology and push the boundaries of genomic research.
Training Opportunities and Workshops
10x Genomics offers a variety of genomics training opportunities to enhance your skills. These workshops cover crucial topics in the field, ensuring you stay at the forefront of genomic research.
Online Training Sessions
Virtual learning options are available for those seeking flexibility. The company provides pre-recorded videos and online resources to support your skill development journey22. These sessions cover topics like single cell gene expression analysis, data quality assessment, and introduction to RNA-seq data analysis22.
In-Person Workshops
For hands-on experience, 10x Genomics conducts in-person workshops in select locations. These events typically run from 1:00 pm to 4:00 pm, lasting about 3 hours23. Workshops focus on spatial transcriptomics, single-cell analysis, and cutting-edge tools like large language models in biomedical research23.
Participants learn to use the Chromium Controller instrument with non-functional reagents in 1-2 hour training sessions24. The company also assists with library preparation for various assays, including single-cell RNAseq, ATAC, and Multiome24.
| Workshop Type | Duration | Key Topics |
|---|---|---|
| Online Sessions | Self-paced | Gene expression, Data quality, RNA-seq analysis |
| In-Person Workshops | 3 hours | Spatial transcriptomics, Single-cell analysis, Language models |
| Instrument Training | 1-2 hours | Chromium Controller usage, Library preparation |
These diverse training options ensure that researchers can develop the necessary skills to leverage 10x Genomics technology effectively in their work.
Contacting 10x Genomics Support
When you need help with 10x Genomics products, reaching out to their customer assistance team is easy. The company offers various ways to contact support, ensuring you get the help you need quickly and efficiently.
How to Reach Out for Help
To contact support, you can email techsupport@10xgenomics.com for technical issues or orders@10xgenomics.com for order-related queries. The help desk is available for users in North America, Europe, and Asia. For Illumina-related questions, reach out to techsupport@illumina.com2526.
What Information to Provide
When contacting the customer assistance team, it’s crucial to provide relevant details about your issue. This helps the support staff address your concerns more effectively. Here’s a list of information to include:
- Product name and version
- Detailed description of the problem
- Any error messages or codes
- Steps you’ve already taken to troubleshoot
- Your contact information
Remember, the more specific you are, the faster the help desk can resolve your issue. If you’re using 10x Genomics Multiome technology, mention which assay workflow you’re using, as this can impact troubleshooting steps.
| Support Type | Contact Method | Response Time |
|---|---|---|
| Technical Support | techsupport@10xgenomics.com | 1-2 business days |
| Order Inquiries | orders@10xgenomics.com | 24-48 hours |
| Illumina Support | techsupport@illumina.com | 1-3 business days |
By providing clear, detailed information when you contact support, you’ll speed up the resolution process and get back to your research faster.
Feedback and Suggestions
10x Genomics values product feedback and user suggestions to drive continuous improvement. Your input shapes the future of single-cell analysis technology.
Importance of User Feedback
User feedback plays a vital role in refining 10x Genomics products. Real-world experiences help identify areas for enhancement. For example, user input led to the development of the V4 kit, which can profile up to 20,000 individual cells per sample with a cell recovery rate of up to 80%27.
How to Submit Suggestions
10x Genomics offers multiple channels for submitting suggestions:
- Online feedback form
- Email support team
- User forums
- Surveys after training sessions
Your insights can lead to significant improvements. For instance, user feedback helped reduce wetting failures in experiments to approximately 1.25% at the HTG Shared Resource28.
| Feedback Type | Impact |
|---|---|
| Product suggestions | New features, improved protocols |
| User experience | Enhanced interfaces, streamlined workflows |
| Technical issues | Bug fixes, improved reliability |
By sharing your experiences and ideas, you contribute to the continuous improvement of 10x Genomics technologies. Your feedback helps create tools that push the boundaries of single-cell analysis, enabling groundbreaking research across diverse fields.
Keeping Up with Updates
10x Genomics consistently enhances its products and software to meet the evolving needs of researchers. Staying informed about product updates and software releases is crucial for maximizing the potential of your research tools.
New Features and Software Releases
The latest Cell Ranger v9.0 release brings significant improvements to the 10x Genomics ecosystem. It now supports GEM-X Universal on-chip multiplexing with 3’v4 and 5’v3 Gene Expression 4-plex assays, enhancing experimental flexibility29. The update also introduces automated cell type annotations for human samples, streamlining data analysis29.
Loupe Browser, another key software, has seen substantial upgrades. Version 8.0 supports visualization and analysis of Visium HD, including bins and exploration of transcript data and microscopy images30. It also enables differential expression and reclustering analysis for unlimited barcodes, providing a progress bar and estimated analysis time for processes30.
Staying Informed Through Newsletters
To keep abreast of these advancements, subscribing to the 10x Genomics newsletter is highly recommended. This subscription ensures you receive timely information about product updates, software releases, and support resources. The newsletter often includes insights into new analytical approaches, such as pseudo-bulk analysis, which can be particularly useful when processing multiple experimental runs31.
By staying connected through these channels, you can leverage the latest features and best practices in your research. Remember, 10x Genomics recommends processing cells in each run separately until they need to be combined, which can help maintain data integrity across your experiments31.
Conclusion: Empowering Your Research
10x Genomics stands at the forefront of research empowerment, offering cutting-edge tools and support for genomics innovation. Their single-cell technologies guide target identification, characterize disease mechanisms, and fuel drug discovery pipelines across various fields including oncology, immunology, and neuroscience32.
The Value of 10x Genomics Support
The company’s commitment to data security is evident in their use of advanced encryption protocols and strict access controls. Their Cloud Instrument Service leverages Amazon Web Services for robust data protection, while their platforms implement AES-256 encryption at rest and TLS in transit33. This dedication to security, combined with their innovative technologies, has contributed to over 8,200 scientific publications, highlighting their significant impact on research advancement.
Encouraging Continued Learning
10x Genomics fosters continued education in genomics through partnerships and expert collaborations. For instance, their partnership with Diamond Age Data Science, their first Data Analysis Partner, brings specialized expertise in analyzing single-cell RNA-sequencing data34. This collaboration enhances the support available to researchers, enabling them to maximize the value of their complex genomic data.
By providing comprehensive support, resources, and cutting-edge technologies, 10x Genomics empowers researchers to push the boundaries of scientific discovery. Their commitment to innovation and continued learning in single-cell, spatial, and in situ genomics positions them as a vital partner in advancing genomic research and driving breakthroughs in understanding complex biological systems.
Q&A
How can I contact 10x Genomics support?
You can reach 10x Genomics technical support via email at support@10xgenomics.com. For orders and customer service inquiries, you can contact orders@10xgenomics.com. Support is available for North America, Europe, and Asia regions.
What products does 10x Genomics offer?
10x Genomics offers innovative platforms for single-cell, spatial, and in situ genomics research. Their product lineup includes Chromium Single Cell for transcriptomic profiling, Visium Spatial for whole transcriptome analysis within morphological context, and Xenium In Situ for spatial imaging with subcellular resolution.
How can I get started with 10x Genomics technology?
10x Genomics offers guidance on initial setup and product selection. They provide a range of solutions including Single Cell Gene Expression Flex, Single Cell Multiome ATAC + Gene Expression, and Single Cell Immune Profiling. The company also offers a beginner’s guide to single-cell RNA-seq to help researchers get started with their technology.
Where can I find product documentation?
10x Genomics provides extensive product documentation including user manuals, protocols, and guides. These resources are available through their Support Hub and can be accessed online.
What should I do if I encounter technical issues?
10x Genomics provides support for troubleshooting common issues such as device malfunctions, software errors, and sample preparation problems. Their technical support team can be reached via email for assistance. The company also offers resources and guides to help users address common problems independently.
Are there any training opportunities available?
Yes, 10x Genomics offers training through their Service Provider Program, which provides access to highly trained scientists and bioinformaticians. While specific details about online sessions and in-person workshops are not provided, the company emphasizes the importance of training and skill development for users of their technologies.
How can I stay updated on new features and software releases?
10x Genomics regularly updates their products and software. Users can stay informed about new features and software releases through the company’s communication channels. The company offers a newsletter subscription for product and support updates, allowing users to stay up-to-date with the latest developments.
Does 10x Genomics offer software solutions for data analysis?
Yes, 10x Genomics provides software solutions for data analysis and interpretation. They offer an overview of their software capabilities and guidance on integration with third-party tools to ensure users can effectively analyze and interpret the complex data generated by their platforms.
Are there resources available for sample preparation?
10x Genomics provides guidance on best practices for sample preparation to ensure high-quality results. They offer tips for obtaining high-quality samples and information on common mistakes to avoid, which is crucial for successful experiments using their single-cell, spatial, and in situ genomics platforms.
Is there a community forum for 10x Genomics users?
While specific forum details are not provided, 10x Genomics fosters a community of users who can engage with each other to share knowledge and solutions. The company encourages collaboration and knowledge sharing among its user base.
